diff --git a/src/Shentun.Peis.Application/SampleGroupDetails/SampleGroupDetailAppService.cs b/src/Shentun.Peis.Application/SampleGroupDetails/SampleGroupDetailAppService.cs
index 6b1bed6..8e29b36 100644
--- a/src/Shentun.Peis.Application/SampleGroupDetails/SampleGroupDetailAppService.cs
+++ b/src/Shentun.Peis.Application/SampleGroupDetails/SampleGroupDetailAppService.cs
@@ -32,17 +32,23 @@ namespace Shentun.Peis.SampleGroupDetails
     public class SampleGroupDetailAppService : ApplicationService
     {
         private readonly IRepository<SampleGroupDetail> _repository;
+        private readonly IRepository<SampleGroup, Guid> _sampleGroupRepository;
         private readonly IRepository<IdentityUser, Guid> _userRepository;
+        private readonly IRepository<Asbitem, Guid> _asbitemRepository;
         private readonly SampleGroupDetailManager _manager;
 
         public SampleGroupDetailAppService(
             IRepository<SampleGroupDetail> repository,
             IRepository<IdentityUser, Guid> userRepository,
-            SampleGroupDetailManager manager)
+            SampleGroupDetailManager manager,
+            IRepository<Asbitem, Guid> asbitemRepository,
+            IRepository<SampleGroup, Guid> sampleGroupRepository)
         {
-            this._repository = repository;
-            this._userRepository = userRepository;
-            this._manager = manager;
+            _repository = repository;
+            _userRepository = userRepository;
+            _manager = manager;
+            _asbitemRepository = asbitemRepository;
+            _sampleGroupRepository = sampleGroupRepository;
         }
 
         /// <summary>
@@ -71,6 +77,24 @@ namespace Shentun.Peis.SampleGroupDetails
             await _manager.CheckAndDeleteAsync(input.SampleGroupId);
             if (input.Details.Any())
             {
+                #region 加限制
+                if (input.Details.Any())
+                {
+                    var isAsbitemEnt = (from sampleGroupDetail in await _repository.GetQueryableAsync()
+                                        join asbitem in await _asbitemRepository.GetQueryableAsync() on sampleGroupDetail.AsbitemId equals asbitem.Id
+                                        join sampleGroup in await _sampleGroupRepository.GetQueryableAsync() on sampleGroupDetail.SampleGroupId equals sampleGroup.Id into sampleGroupTemp
+                                        from sampleGrouphaveEmpty in sampleGroupTemp.DefaultIfEmpty()
+                                        where input.Details.Select(s => s.AsbitemId).Contains(sampleGroupDetail.AsbitemId)
+                                        select new
+                                        {
+                                            asbitemName = asbitem.DisplayName,
+                                            sampleGroupName = sampleGrouphaveEmpty != null ? sampleGrouphaveEmpty.DisplayName : ""
+                                        }).FirstOrDefault();
+                    if (isAsbitemEnt != null)
+                        throw new UserFriendlyException($"组合项目{isAsbitemEnt.asbitemName}已在{isAsbitemEnt.sampleGroupName}条码分组中，不允许添加");
+                }
+                #endregion
+
                 List<SampleGroupDetail> sampleGroupDetails = new List<SampleGroupDetail>();
                 foreach (var details in input.Details)
                 {